aSchool of Chemistry, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
bNanomaterials & Solar Energy Conversion Lab, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, Tamil Nadu, India
Available online 19 September 2008.
The interaction between colloidal TiO2 and human serum albumin (HSA) was studied by using absorption, fluorescence and synchronous fluorescence spectroscopic measurements. Colloidal TiO2 effectively quenched the intrinsic fluorescence of HSA. The number of binding sites (n) and apparent binding constant (K) were calculated by fluorescence quenching data. The interaction between colloidal TiO2 and HSA occurs through static quenching and conformational changes of HSA were observed.
Keywords: Colloidal TiO2; Fluorescence quenching; HSA
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